Clinical Laboratory
Counting chambers

Precision Measuring Instruments from BRAND

Counting chambers serve to determine the number of particles per volume unit of a liquid. The particles (e.g. leucocytes, erythrocytes, thrombocytes, bacteria, fungus spores, pollen) are visually counted under a microscope.

BLAUBRAND® counting chambers are precision measuring instruments. They conform to DIN 12847 and to "Eichordnung" the German Federal Weights and Measures Regulations.

 
Description of functional characteristics

The microscope slide sized base plate is made of special optical glass. Milled grooves divide the surface into two large fields (outside) and three nar-
row ridges (inside). The two outer fields are for inscriptions, whereas the ridges are ground and polished. The central ridge (= chamber bottom) has two engraved sets of rulings for counting, separated by a groove. Generally the chamber bottom on the central ridge is 0.1 mm lower 
(= chamber depth) than the two outer ridges. Hence, when a cover glass 
is placed on top, there is a gap of

0.1 mm between the glass and the central ridge. The lateral boundaries of the volume to be counted are formed by the imaginary planes projected vertically onto the boundary lines of the ruling. 

Cleaning 
For cleaning, we recommend the disinfectant cleaner Mucocit®-T.

Pack quantities for all counting chambers:
1 Counting chamber, complete with 2 haemacytometer cover glasses, in transparent plastic box.

BRAND counting chamber and haemacytometer cover glasses are CE-marked according to IVD-Directive 98/79 EC.
Equation for particle determination (for general use)

 

Particles per µl Volumen =                              Counted particles                             
                                       Counted surface (mm2) · Chamber depth  (mm) · Dilution

 

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