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Technical information
Disinfection/Sterilization

Disinfection 

Laboratory instruments that have come into contact with infectious material or genetically modified organisms must be disinfected prior to reuse / disposal; i.e., they must be brought to a condition in which they no longer pose a risk. Therefor laboratory instruments can be treated with disinfecting detergents for example. If necessary and suitable, the items may subsequently be sterilized (autoclaved). 

Steam sterilization 
Steam sterilization (autoclaving) is defined as the destruction or irrever-sible inactivation of all reproducible microorganisms under exposure to saturated steam at 121 °C (2 bar), according to DIN EN 285. For correct sterilization procedure, including biological security, please contact your sterilization officer. 

 

The following points must be observed: 

  • Efficient steam sterilization is assured only if the steam is saturated and has unrestricted access to all contaminated areas. 
  • To prevent pressure build-up, con-tainers or vessels must always be open. 
  • Contaminated reusable labware must be cleaned thoroughly before steam sterilization. Otherwise, residue will bake on during sterilization and microorganisms may not be effec-tively destroyed if they are protected by the residue. Furthermore, any adhering chemical residues may damage the surfaces due to the high temperatures.
  • Not all plastics are resistant to steam sterilization. Polycarbonate, e.g., will lose its strength. Polycarbo-nate centrifuge tubes cannot be steam sterilized.  
  • During sterilization (autoclaving), plastic labware in particular should not be mechanically stressed (e.g., do not stack). Thus, to avoid shape deformation, beakers, flasks, and graduated cylinders should be autoclaved in an upright position.

Thermal resistance 

All reusable BLAUBRAND® and SILBERBRAND volumetric instruments can be heated up to 250 °C in a drying cabinet or a sterilizer, without any subsequent volume deviations. However, as with all glass instru-ments, irregular heating or sudden temperature changes produce thermal stresses which may result in breakage.

Therefore: 

  • Always place glass instruments into a cold drying cabinet or sterilizer; then heat slowly. 
  • At the end of the drying or sterilizing period, allow instruments to cool off slowly inside the switched-off oven. 
  • Do not heat up volumetric instru-ments on a hot plate. 
  • Pay special attention to the maxi-mum operating temperatures of plastic instruments.