Grupo de productos: Productos Life Science

Cells seeded in the insert detaches from the membrane. What could be done?

When plating cells, make sure that medium levels inside the well is lower than in the lumen of insert. This prevents a medium flux through the membrane pores from the well into the inserts that is oppositly directed to the settlement of the cells. Some cell types also need special coatings or media supplements or even preconditioned media to attach properly.