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Dispensing

with bottle-top dispensers

Definition of 'dispensing':

The term 'dispensing' is understood to mean the discharge of defined quantities. For easy, rapid and precise dispensing of reagents, bottle-top dispensers are widely employed to replace poured transfers into graduated cylinders. They can be mounted directly on commercial laboratory bottles, either directly or by means of adapters. It is no longer necessary to transfer or decant chemicals. Serial dispensing in particular is greatly facilitated.

Functional principle of bottle-top dispensers

By an upward movement of the piston, the preset amount of liquid is aspirated from the reagent bottle into the dispenser cylinder. By the subsequent downward movement of the piston, the liquid is released through a valve system and the discharge tube. There is no need to set a meniscus or to observe a waiting time.

Dispensing

Bottle-top dispensers with floating pistons, e.g., Dispensette® from BRAND

Dispensette S

For dispensing aggressive reagents, e.g., concentrated acids such as H3PO4, H2SO4, bases like NaOH, KOH, saline solutions, as well as many organic solvents: Dispensette® S

Dispensette Organic

For dispensing organic solvents, such as chlorinated and fluorinated hydrocarbons (e.g., trichlorotrifluoroethane and dichloromethane), or acids such as concentrated HCl and HNO3, as well as for trifluoroacetic acid (TFA), tetrahydrofuran (THF) and peroxides: Dispensette® S Organic

Dispensette S TA

For dispensing HF we recommend the use of the Dispensette® S Trace Analysis with a platinum-iridium valve spring.

Dispensette® S, Digital, DE-M, 1-10ml, with recirculation valve

Whether you are dispensing solvents, acids, alkalis or saline solutions – the Dispensette® S bottle-top dispenser makes it easy, safe and efficient.With both the Dispensette® S and Dispensette® S Organic, you will always have the right dispenser at hand for a wide range of media. For efficient volume adjustment, you can choose between digital and analog adjustment, or fixed volume. Do you dispense long series, sterile applications or moisture-sensitive media? With numerous accessory options, the Dispensette® S also offers efficient solutions for special applications. Dispensette® S (red color-code): Ideal for dispensing aggressive reagents, including concentrated bases and acids such as H3PO4, H2SO4 (except e.g., HCl, HNO3 und HF), saline solutions, as well as many organic solvents. Digital design: Especially easy to read digital display. The selected dosed volume can be adjusted precisely and is easy to reproduce. Easy calibration technology for adjustment in seconds without tools. Automatic designation if factory default changed. Einsatzgrenzen: Operating temperature from +15 °C to +40 °C (from 59 °F to 104 °F) of instrument and reagent Vapor pressure up to max. 600 mbar. Aspirate slowly above 300 mbar, in order to prevent the liquid from boiling Kinematic viscosity up to 500 mm² /s (dynamic viscosity [mPas] = kinematic viscosity [mm² /s] x density [g/cm³) Density up to 2.2

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Dispensette® S, Analog-adjustable, DE-M, 0.1-1ml, without recirculation valve

Whether you are dispensing solvents, acids, alkalis or saline solutions – the Dispensette® S bottle-top dispenser makes it easy, safe and efficient.With both the Dispensette® S and Dispensette® S Organic, you will always have the right dispenser at hand for a wide range of media. For efficient volume adjustment, you can choose between digital and analog adjustment, or fixed volume. Do you dispense long series, sterile applications or moisture-sensitive media? With numerous accessory options, the Dispensette® S also offers efficient solutions for special applications. Dispensette® S (red color-code): Ideal for dispensing aggressive reagents, including concentrated bases and acids such as H3PO4, H2SO4 (except e.g., HCl, HNO3 und HF), saline solutions, as well as many organic solvents. Analog-adjustable Model: Rapid and safe volume setting with new internal scalloped track. Simple calibration adjustment with a supplied tool. Einsatzgrenzen: Operating temperature from +15 °C to +40 °C (from 59 °F to 104 °F) of instrument and reagent Vapor pressure up to max. 600 mbar. Aspirate slowly above 300 mbar, in order to prevent the liquid from boiling Kinematic viscosity up to 500 mm² /s (dynamic viscosity [mPas] = kinematic viscosity [mm² /s] x density [g/cm³) Density up to 2.2

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Dispensette® S Organic, Digital, DE-M, 1-10ml, with recirculation valve

Whether you are dispensing solvents, acids, alkalis or saline solutions – the Dispensette® S bottle-top dispenser makes it easy, safe and efficient.With both the Dispensette® S and Dispensette® S Organic, you will always have the right dispenser at hand for a wide range of media. For efficient volume adjustment, you can choose between digital and analog adjustment, or fixed volume. Do you dispense long series, sterile applications or moisture-sensitive media? With numerous accessory options, the Dispensette® S also offers efficient solutions for special applications. Dispensette® S Organic (yellow color-code): Ideal for dispensing organic solvents, including chlorinated and fluorinated hydrocarbons (e.g., trichlorofluoroethane and dichlormethane), acids like concentrated HCl and HNO3 (except HF), trifluoroacetic acid (TFA), tetrahydrofuran (THF) and peroxides. Digital design: Especially easy to read digital display. The selected dosed volume can be adjusted precisely and is easy to reproduce. Easy calibration technology for adjustment in seconds without tools. Automatic designation if factory default changed. Einsatzgrenzen: Operating temperature from +15 °C to +40 °C (from 59 °F to 104 °F) of instrument and reagent Vapor pressure up to max. 600 mbar. Aspirate slowly above 300 mbar, in order to prevent the liquid from boiling Kinematic viscosity up to 500 mm² /s (dynamic viscosity [mPas] = kinematic viscosity [mm² /s] x density [g/cm³) Density up to 2.2

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Dispensette® S Trace Analysis, DE-M, PT/IR, 1-10 ml, with recirculation valve

The Dispensette® S Trace Analysis provides outstanding performance for precise volume dispensing of high-purity media for trace analysis. Also suitable for HF with platinum-iridium valve spring. Plastics in contact with media consist of high-purity materials such as PTFE, ETFE, FEP, and PFA. The purest sapphire is used for replaceable valves. Depending on the application, platinum-iridium or tantalum are available as spring materials. A field-tested cleaning process before use in trace analysis is described in the operating manual. If contamination of the bottle contents must be avoided, we recommend using the device without the recirculation valve. The 45 mm standard thread plus the included adapters fit most common lab bottles. The valve block can be rotated 360° so that the bottle label always faces the user for safety. Telescoping filling tube adjusts to different bottle sizes. Easy disassembly for replacement of the dispensing cartridge.

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Documents download

Dispensette®

Testing instructions (SOP) | pdf | 681 KB

Dispensette® S

Operating Manual | pdf | 13 MB

Dispensette® S

Brochure | pdf | 1 MB

Dispensette® S

Selection chart | pdf | 33 KB

Dispensette® S - Care and use

Videos | mp4 | 25 MB

Dispensette® S - serial dispensing

Videos | mp4 | 27 MB

Dispensette® S - Thread adapters

Videos | mp4 | 26 MB

Dispensette® S Accessories

Videos | mp4 | 6 MB

Dispensette® S Cleaning

Videos | mp4 | 42 MB

Dispensette® S Introduction

Videos | mp4 | 3 MB

Dispensette® S Start up

Videos | mp4 | 19 MB

Flask adapter

Selection chart | pdf | 442 KB

Liquid Handling with Bottle-Top Instruments

Application Note | pdf | 734 KB

Seripettor

Bottle-top dispensers with wiping-seal piston, e.g., seripettor® from BRAND

The design of the system allows a replacement of the complete dispensing cartridge. The somewhat higher operating forces dur-ing filling are minimized by a spring with automatic lifting action. The present example reflects a competitively-priced dispenser for simple dispensing tasks in the volume range of 0.2 to 25 ml.

 

Range of application and materials

 

The range of application in-cludes the daily routine dispens-ing of bases, acids in low con-centration, biological buffers, cell culture media, biological detergents and polar solvents.

The seripettor® pro dispenser is suitable for dispensing acids such as concentrated HCl, po-lar solvents (e.g., acetone) and UV-sensitive media. In contrast to the seripettor® dispenser, valves made from chemically resistant materials are used in this instrument.

seripettor®, 1-10 ml

seripettor® and seripettor® pro are innovative bottle-top dispensers from BRAND with a special design. A lifting spring provides automatic filling. When the piston seal is worn out, the entire dispensing unit can be exchanged by the user quickly and easily. Operating range: seripettor® : Aqueous solutions: Routinely used biological buffer solutions and detergents, antifoaming agents, culture media, vitamin solutions and so on can be dispensed. Hydrogen peroxide can also be dispensed. Agar culture media can be dispensed at up to a max. of 60 °C. Acids: Weak, dilute or non-oxidizing acids can be dispensed. Alkaline solutions: The components of the instrument are compatible with alkaline solutions, such as NaOH, KOH and ammonia. Polar solvents: e.g., polar solvents such as ethanol, methanol, acetylacetone, etc. Einsatzgrenzen: Use between +15°C and +40°C of instrument and reagent (seripettor®: agar culture medium up to max. 60 °C) Vapor pressure up to 500 mbar Density up to 2.2 g/cm³ Kinematic viscosity*: 2 ml instrument: 300 mm² /s; 10 ml instrument: 150 mm² /s; 25 ml instrument: 75 mm² /s. * dynamic viscosity [mPas] = kinematic viscosity [mm² /s] x density

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seripettor® pro, 0.2-2 ml

seripettor® and seripettor® pro are innovative bottle-top dispensers from BRAND with a special design. A lifting spring provides automatic filling. When the piston seal is worn out, the entire dispensing unit can be exchanged by the user quickly and easily. Uses of seripettor® pro: Aqueous solutions: Biological buffer solutions and detergents, anti-foaming agents, culture media, vitamin solutions, etc. can be dosed in everyday routines. Lyes: such as NaOH, KOH and ammonia. Polar solvents: polar solvents such as ethanol, methanol, acetylacetone, Acetone etc. Acids: such as concentrated HCI, glacial acetic acid. UV-sensitive media Einsatzgrenzen: Use between +15°C and +40°C of instrument and reagent (seripettor®: agar culture medium up to max. 60 °C) Vapor pressure up to 500 mbar Density up to 2.2 g/cm³ Kinematic viscosity*: 2 ml instrument: 300 mm² /s; 10 ml instrument: 150 mm² /s; 25 ml instrument: 75 mm² /s. * dynamic viscosity [mPas] = kinematic viscosity [mm² /s] x density

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Documents download

Flask adapter

Selection chart | pdf | 442 KB

seripettor®

Operating Manual | pdf | 11 MB

seripettor®

Selection chart | pdf | 49 KB

seripettor® pro

Brochure | pdf | 869 KB

seripettor® pro

Brochure | pdf | 1 MB

Repetitive pipetting – perform long series quickly and reproducibly

With repetitive pipettes (also known as steppers), a single aspirated volume can be dispensed precisely step by step. This allows long test series to be processed reproducibly and quickly and simplifies complex sequences providing both high efficiency and accuracy. Due to the positive displacement principle used, even handling of problematic liquids is possible.

The positive displacement principle

The positive displacement principle is often used for repetitive pipettes.

In contrast to air cushion systems (e.g., in micropipettes), the piston of positive displacement systems has direct contact with the liquid to be pipetted. When aspirating, the piston draws in the liquid by creating a vacuum and then wipes the walls of the tip clean during dispensing

 

Advantage  

This operating principle ensures precisely reproducible results regardless of the liquid and without the influence of an air cushion. Viscous liquids, highly volatile solutions, or foaming media can be accurately and easily dispensed.

Manual or electronic – the right model for every application

The manual repetitive pipette

e.g., HandyStep® S from BRAND                                                    

Routine work, production or long series can be handled efficiently with a manual repetitive pipette. With the simple setting wheel, the most common volumes can be set easily and quickly.

  • Robust and durable
  • Quickly adjustable volume
  • Easy to use and compatible with tips from other manufacturers

 

The electronic repetitive pipette

e.g. HandyStep® touch and HandyStep® touch S from BRAND

 

Complex processes and extensive protocols can be efficiently implemented with electronic repetitive pipettes. The electronic volume adjustment ensures high flexibility in volume settings. The touch display and range of functions enable almost any test procedure.

 

  • Simple: Touchscreen operation similar to a smartphone
  • Easy: Electronic tip ejection
  • Efficient: Recognition of tip size of BRAND PD-Tips II, but also compatible with tips from other manufacturers

The right choice

With so many possibilities, it can be difficult to make the right choice for your applications. Positive displacement or air cushion? Manual or electronic? Simple for standard applications or extended functions for complex processes?

The range of devices is just as diverse as the applications.

Our Application Notes will help you make the right choice and find the perfect device for your application.

HandyStep® touch, DE-M, inclusive universal AC adapter and shelf/rack mount

Repetitive pipettes are the first choice when it comes to efficient dispensing of long series. Positive displacement pipettes are often the right solution for pipetting viscous media. The new stepper HandyStep® touch from BRAND combines both features. With the touchscreen display, you conveniently swipe back and forth between functions and always have an overview of the essential information on your work steps. Operation is easy, even with protective gloves on. And speaking of easy, the days of manually changing tips are over. On the HandyStep® touch, lightly tapping on the Eject icon on the display is enough to eject the tip. The HandyStep® touch repetitive pipette automatically recognizes the volume of the new BRAND PD- Tips II. This saves time and reduces the risk of error. Tips from many other manufacturers can also be used, providing maximum flexibility. Charging is easy and efficient: simply place the HandyStep® touch in the inductive charging stand and the battery automatically starts charging. Overview - Touchscreen operation with intuitive menu navigation in English, German, Spanish, French and Chinese - HandyStep® touch with the features multi-dispensing, auto-dispensing and pipetting; HandyStep® touch S with the additional features sequential dispensing, multi-aspiration and titration - Automatic size detection of BRAND PD-Tips II - Compatible with PD-Tips prior to the PD-Tips II generation and with tips from many other manufacturers: for example, Encode™ tips, Repet-Tips®, Combitips®, Combitips® plus, Combitips® advanced (the above-mentioned brands are property of their respective owners) - Automatic tip ejection - Variable volume adjustment from 1.0 μl to 50 ml - Easy to clean thanks to smooth surface without grooves and edges - Inductively rechargeable Einsatzgrenzen: Operating temperature of instrument and reagent should be between +15 °C and +40 °C (59 °F to 104 °F) (other temperatures upon request) Vapor pressure up to 500 mbar Viscosity: At 50 ml PD-Tip: 20 mPa s; at 5 ml PD-Tip: 260 mPa s; at 1,25 ml PD-Tip: 977 mPa

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Repetitive pipette HandyStep® S

HandyStep® S – the repetitive pipette for so many applications! Ideal for routine or research applications in diagnostics, molecular biology, environmental analysis, and many more.HandyStep® S and PD-tips work on the direct displacement principle. This offers the highest-precision dispensing of liquid media with high viscosity, high density, or high vapor pressure. Direct displacement permits contamination-free operation, since no aerosols are formed. More data and facts: increased chemical resistance through innovative plastic materials easy tip mounting – PD-tip is now simply inserted from below dispensed volume range from 2 µl to 5 ml up to 49 dispensing steps weighs only 108 g suitable for use with BRAND PD-tips, Encode™ tips, Repet tips, Combitips®, Combitips® plus and other compatible dispenser tips. Einsatzgrenzen: Operating temperature of instrument and reagent should be between +15 °C and +40 °C (59 °F to 104 °F) (other temperatures upon request) Vapor pressure up to 500 mbar Viscosity: At 50 ml PD-Tip: 20 mPa s; at 5 ml PD-Tip: 260 mPa s; at 1,25 ml PD-Tip: 977 mPa

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Documents download

Air displacement pipette or repetitive pipette?

Application Note | pdf | 1 MB

HandyStep® touch

Operating Manual | pdf | 6 MB

HandyStep® touch

Brochure | pdf | 2 MB

HandyStep® touch

Selection chart | pdf | 367 KB

HandyStep® touch - auto dispensing

Videos | mp4 | 9 MB

HandyStep® touch - multi dispensing

Videos | mp4 | 8 MB

HandyStep® touch - pipetting modes

Videos | mp4 | 6 MB

HandyStep® touch - safe dispensing

Videos | mp4 | 6 MB

HandyStep® touch with PD-Tips II Accuracy table

Selection chart | pdf | 83 KB

HandyStep® touch/HandyStep® electronic/HandyStep® S

Testing instructions (SOP) | pdf | 1 MB

PD-Tip Adapter

Videos | mp4 | 14 MB

Repetitive pipette HandyStep® touch

Videos | mp4 | 7 MB

Volumetric Measurement

Brochure | pdf | 4 MB

HandyStep® touch

Brochure | pdf | 2 MB

HandyStep® touch with PD-Tips II Accuracy table

Selection chart | pdf | 83 KB

Safety always comes first!

When choosing a bottle-top dispenser, the safety features of the instrument should be kept in mind. For example, does it reduce the risk of injury due to glass breakage? How does it avoid accidental splashing when the instrument is primed? How is contact with the medium minimized when the dispensing tube is closed? Likewise, the suitability of the dispenser for the medium to be dispensed should be checked by the user. Information about this can generally be found in the operating manual in the chapter 'Function and limitations of use'. When in doubt, contact the manufacturer directly. Information on maintenance and monitoring of measuring instruments are also found in the operating manual.

Calibration

Monitoring of measuring instruments / calibration

In regard to the monitoring of measuring instruments according to ISO and GLP  guidelines, the accuracy of volumetric instruments should be checked regularly and recalibrated if necessary.