Pipetting with micropipettes

Pipetting with air interface pipettes

The up and down movement of the piston inside the pipette shaft creates a negative or positive pressure of the air column. As a result, liquid is either aspirated into the tip or expelled from it. The air column (air interface) keeps the liquid separated from the piston.

Benefits

There is no wetting of the instrument; the liquid only enters the tip. Tips are used only once, which eliminates any carry-over. This is particularly important for applications where sterile conditions are required, or no carry-over is allowed.

Calibration

In regard to the monitoring of measuring instruments according to ISO and GLP guidelines, the accuracy of volumetric instruments should be calibrated regularly (i.e. checked) and adjusted if necessary.

Manual single-channel pipettes

e.g., Transferpette® S from BRAND

In routine labwork and in research, precision and functionality

are the standards to expect from air interface piston-operated pipettes today.

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Aspirate reagent

Press pipetting button to the first stop. Hold the pipette vertically and immerse the tip into the liquid.
Allow the pipetting button to slowly slide back so that the liquid will be aspirated.

Discharge reagent

Place the pipette tip against the wall of the vessel. Hold the pipette at an angle of 30-45° relative to the container wall and press the pipetting button
slowly to the first stop and hold it down.
The blow-out stroke empties the tip completely: press the pipetting button down to the second stop and wipe the pipette tip on the vessel wall.

Eject tip

Press the tip ejector.

Transferpette® S, 0.1-1 µl, adjustable volume, D-1, DE-M

The Transferpette® S air displacement pipette allows you to work efficiently and ergonomically with both small and large volumes. Low operating forces and a short stroke ensure relaxed…

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Transferpette® S -8, 10-100 µl, adjustable volume, M8-100, DE-M

The Transferpette® S air displacement pipette allows you to work efficiently and…

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Transferpette® S -12, 5-50 µl, adjustable volume, M12-50, DE-M

The Transferpette® S air displacement pipette allows you to work efficiently and…

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Electronic single- and multichannel pipettes

e.g., Transferpette® electronic from BRAND

Pressing the pipetting button starts the aspiration or discharge mechanisms (incl. blow-out). The pipette’s piston is moved by a motor, aspiration and discharge are controlled by a microprocessor. Various pipetting programs can be selected with the control keys.

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Aspirate reagent

Immerse the tip in the reagent and press the pipetting button once – the set volume will be aspirated.

Discharge reagent

Place the pipette tip against the wall of the vessel. Hold the pipette at an angle of 30-45° relative to the container wall and press the pipetting button again – the liquid will be discharged. The blow-out takes place automatically! During this process, wipe the tip on the vessel wall.

Eject tips

Press the tip ejector.