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Pipetting

Simple and efficient

Tips and tricks for pipetting

Pipetting is the accurate one-time uptake and delivery of liquids. An air interface pipette is used for pipetting aqueous liquids in the microliter to milliliter range. It operates by the air interface principle.

Pipetting with air interface pipettes

The up and down movement of the piston inside the pipette shaft creates a negative or positive pressure of the air column. As a result, liquid is either aspirated into the tip or expelled from it. The air column (air interface) keeps the liquid separated from the piston.

Benefits
There is no wetting of the instrument; the liquid only enters the tip. Tips are used only once, which eliminates any carry-over. This is particularly important for applications where sterile conditions are required, or no carry-over is allowed.
Calibration
In regard to the monitoring of measuring instruments according to ISO and GLP guidelines, the accuracy of volumetric instruments should be calibrated regularly (i.e. checked) and adjusted if necessary.
Pipette

Manual single-channel pipettes

e.g., Transferpette® S from BRAND

 

In routine labwork and in research, precision and functionality

are the standards to expect from air interface piston-operated pipettes today.

 

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Aspirate reagent

  1. Press pipetting button to the first stop. Hold the pipette vertically and immerse the tip into the liquid.
  2. Allow the pipetting button to slowly slide back so that the liquid will be aspirated.

Discharge reagent

  1. Place the pipette tip against the wall of the vessel. Hold the pipette at an angle of 30-45° relative to the container wall and press the pipetting button
    slowly to the first stop and hold it down.
  2. The blow-out stroke empties the tip completely: press the pipetting button down to the second stop and wipe the pipette tip on the vessel wall.

Eject tip

 

Press the tip ejector.

Transferpette
Transferpette
Transferpette

Our product selection

Transferpette® S, 0.1-1 µl, adjustable volume, D-1, DE-M

The Transferpette® S air displacement pipette allows you to work efficiently and ergonomically with both small and large volumes. Low operating forces and a short stroke ensure relaxed pipetting, even during long pipetting series. Due to its perfect ergonomics, the Transferpette® S provides a comfortable grip in any hand position, whether you are right- or left-handed, or have big or small hands. Changing your grip is not necessary, since the microliter pipette provides true one-hand operation: set the volume, pipette, eject the tip.The volume-change protection is also easy to operate with gloves on and protects your set volume against accidental adjustment. The Transferpette® S also features Easy Calibration technology, which makes calibration a breeze. Calibration can be carried out without tools. A compatibility table shows which tips fit which device, so that you always order the correct pipette tips.Einsatzgrenzen: Operating temperature of instrument and reagent should be between +15 °C and +40 °C (59 °F to 104 °F) (other temperatures upon request) Vapor pressure up to 500 mbar Viscosity: 260 mPa s For viscous media, the speed must be adjusted if necessary.

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Transferpette® S -8, 10-100 µl, adjustable volume, M8-100, DE-M

The Transferpette® S air displacement pipette allows you to work efficiently and ergonomically with both small and large volumes. Low operating forces and a short stroke ensure relaxed pipetting, even during long pipetting series. Due to its perfect ergonomics, the Transferpette® S provides a comfortable grip in any hand position, whether you are right- or left-handed, or have big or small hands. Changing your grip is not necessary, since the microliter pipette provides true one-hand operation: set the volume, pipette, eject the tip.The volume-change protection is also easy to operate with gloves on and protects your set volume against accidental adjustment. The Transferpette® S also features Easy Calibration technology, which makes calibration a breeze. Calibration can be carried out without tools. A compatibility table shows which tips fit which device, so that you always order the correct pipette tips.Einsatzgrenzen: Operating temperature of instrument and reagent should be between +15 °C and +40 °C (59 °F to 104 °F) (other temperatures upon request) Vapor pressure up to 500 mbar Viscosity: 260 mPa s For viscous media, the speed must be adjusted if necessary.

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Transferpette® S -12, 5-50 µl, adjustable volume, M12-50, DE-M

The Transferpette® S air displacement pipette allows you to work efficiently and ergonomically with both small and large volumes. Low operating forces and a short stroke ensure relaxed pipetting, even during long pipetting series. Due to its perfect ergonomics, the Transferpette® S provides a comfortable grip in any hand position, whether you are right- or left-handed, or have big or small hands. Changing your grip is not necessary, since the microliter pipette provides true one-hand operation: set the volume, pipette, eject the tip.The volume-change protection is also easy to operate with gloves on and protects your set volume against accidental adjustment. The Transferpette® S also features Easy Calibration technology, which makes calibration a breeze. Calibration can be carried out without tools. A compatibility table shows which tips fit which device, so that you always order the correct pipette tips.Einsatzgrenzen: Operating temperature of instrument and reagent should be between +15 °C and +40 °C (59 °F to 104 °F) (other temperatures upon request) Vapor pressure up to 500 mbar Viscosity: 260 mPa s For viscous media, the speed must be adjusted if necessary.

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Electronic single- and multichannel pipettes

e.g., Transferpette® electronic from BRAND

Pressing the pipetting button starts the aspiration or discharge mechanisms (incl. blow-out). The pipette’s piston is moved by a motor, aspiration and discharge are controlled by a microprocessor. Various pipetting programs can be selected with the control keys.

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Aspirate reagent

 

Immerse the tip in the reagent and press the pipetting button once – the set volume will be aspirated.

Discharge reagent

 

Place the pipette tip against the wall of the vessel. Hold the pipette at an angle of 30-45° relative to the container wall and press the pipetting button again – the liquid will be discharged. The blow-out takes place automatically! During this process, wipe the tip on the vessel wall.

Eject tips

 

Press the tip ejector.

Transferpette
Transferpette
Transferpette

Our product selection

Transferpette® electronic, DE-M, 0.5-10 µl, power adapter Europe (100-240V/50-60Hz)

Transferpette® electronic pipettes combines the globally proven properties of mechanical piston operated air displacement pipettes by BRAND with the advantages of an electronic device.Features:The pipette shaft can be unscrewed and is entirely autoclavable (121 °C).Five convenient programs: pipetting, reverse pipetting, mixing, GEL-Electrophoresis and dispensing.Easy Calibration-Technique for making quick adjustments of the Transferpette® electronic, without tools.Einsatzgrenzen:Operating temperature of instrument and reagent should be between +15 °C and +40 °C (59 °F to 104 °F) (other temperatures upon request)Vapor pressure up to 500 mbarViscosity: 260 mPa sFor viscous media, the speed must be adjusted if

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Transferpette® -8 electronic, DE-M, 10-200 µl, power adapter Europe (100-240V/50-60Hz)

Transferpette® electronic pipettes combines the globally proven properties of mechanical piston operated air displacement pipettes by BRAND with the advantages of an electronic device. Features: The pipette shaft can be unscrewed and is entirely autoclavable (121 °C). Functions: pipetting, reverse pipetting, mixing, dispensing, up to 300 µl also GEL-Electrophoresis. Easy Calibration-Technique for making quick adjustments of the Transferpette® electronic, without tools.   Einsatzgrenzen: Operating temperature of instrument and reagent should be between +15 °C and +40 °C (59 °F to 104 °F) (other temperatures upon request) Vapor pressure up to 500 mbar Viscosity: 260 mPa s For viscous media, the speed must be adjusted if

view product

Transferpette® -12 electronic, DE-M, 15-300 µl, power adapter Europe (100-240V/50-60Hz)

Transferpette® electronic pipettes combines the globally proven properties of mechanical piston operated air displacement pipettes by BRAND with the advantages of an electronic device. Features: The pipette shaft can be unscrewed and is entirely autoclavable (121 °C). Functions: pipetting, reverse pipetting, mixing, dispensing, up to 300 µl also GEL-Electrophoresis. Easy Calibration-Technique for making quick adjustments of the Transferpette® electronic, without tools. Einsatzgrenzen: Operating temperature of instrument and reagent should be between +15 °C and +40 °C (59 °F to 104 °F) (other temperatures upon request) Vapor pressure up to 500 mbar Viscosity: 260 mPa s For viscous media, the speed must be adjusted if

view product

Documents download

Air displacement pipette or repetitive pipette?

Application Note | pdf | 1 MB

Influences on volume in air-displacement pipettes

Application Note | pdf | 2 MB

Monitoring test equipment for air displacement pipettes

Application Note | pdf | 680 KB

Pipette Tips and Filter Tips

Selection chart | pdf | 327 KB

Transferpette®

Testing instructions (SOP) | pdf | 1 MB

Transferpette® electronic

Operating Manual | pdf | 4 MB

Transferpette® electronic

Brochure | pdf | 1 MB

Transferpette® electronic - Advanced pipetting

Videos | mp4 | 16 MB

Transferpette® electronic - General pipetting

Videos | mp4 | 10 MB

Transferpette® electronic - reference movement

Videos | mp4 | 9 MB

Pipette Tips and Filter Tips

Selection chart | pdf | 462 KB