Production of in-vitro test systems with BRAND Inserts 2in1
Welcome to the future of cell cultivation with the BRAND Insert 2in1. Our innovative inserts offer the flexibility and reproducibility you need to take your lab work to the next level and replacing animal testing by in-vitro assays.
Why choose BRAND Inserts 2in1?
Versatile Use
The BRAND Insert 2in1 can be used hanging or standing in your multiwell plates without the need for additional support plates. This allows for easy handling and integration into your existing lab setup.
Compatibility
Perfectly tailored to ANSI/SLAS standard multiwell plates, our inserts fit 6-, 12-, and 24-well plates.
Cell-friendly
Equipped with membranes specifically treated for cell culture (cellGrade™ plus), our inserts promote optimal cell growth and survival. Via its protein-like structure, the cellGrade™ plus surface provides optimal conditions for cultivation of cell lines and primary cells.
Proven Quality for Regulatory Testing
Studies confirm that the BRAND Insert 2in1 supports the cultivation of reconstructed human epidermis (RhE) for skin corrosion testing in compliance with OECD TG 431. By enabling reliable, high-reproducibility data for regulatory safety assessments, it plays a key role in reducing animal testing.
Applications
- Skin corrosion
- Toxicity assessments
- Barrier and transportation studies (TEER)
- Polarity testing
- Transmigration and invasion assays
- Tissue engineering
- Co-cultivation
- Cell polarization studies
Technical advantages
What researchers are saying
BRAND ® Insert 2in1 supports the cultivation of Reconstructed Human Epidermis
Reconstructed Human Epidermis (RhE) is used as an alternative in vitro test system partially able to replace tests on laboratory animals and provide data that may be more predictive for humans when compared to animal testing.
Studies using Inserts 2in1
Organ on a chip, reconstructed tissue
Phenion® full thickness human skin model
In vitro eye irritation testing
SEPT9 and EPLIN modulates the adhesion and migration of human fibroblasts
Development of a microphysiological skin-liver-thyroid Chip3 model
Environmentally Controlled Microfluidic System
Development of a microphysiological skin-liver-thyroid Chip3 model
Full thickness 3D in vitro conjunctiva model
Exploring the inhibitory potential of the antiarrhythmic drug amiodarone
New Indole-3-Propionic Acid and 5-Methoxy-Indole Carboxylic Acid
Human α-Defensin-6 Neutralizes Clostridioides difficile Toxins
Preparation of Phosphorescent Eu2+, Dy3+-Doped Strontium Aluminate Nanoparticles
Growth in 3D Skin Infection Models
In-situ tear fluid dissolving nanofibers
In vitro assessment of P-gp and BCRP transporter-mediated drug–drug interactions
Learn more
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Frequently asked questions
Application and Compatibility
Q: Can the 2in1 inserts be used both hanging and standing?
A: Yes, the BRAND 2in1 inserts can be flexibly positioned either hanging or standing, thanks to their stand feet and hooks.
Q: Which plate formats are compatible with the 2in1 inserts?
A: The 2in1 inserts are suitable for 24-well, 12-well, and 6-well plates, as well as culture trays.
Cell Growth and Surface
Q: How is uniform and controlled cell growth achieved?
A: The cellGradeTM plus surface optimally supports cell attachment due to its protein-like structure, without the need for additional coatings.
Q: Can the cellGradeTM plus surface be further modified?
A: Yes, the surface can be adjusted with additional biological coatings such as laminin or collagen if needed.
Technical Specifications
Q: What membrane materials and pore sizes are available?
A: PC and PET membranes are available with pore sizes of 0.4 μm, 1.0 μm, 3.0 μm, and 8.0 μm.
Q: What is the growth area of the inserts?
A: The growth area on the membrane is 59 mm².
Applications
Q: For which types of cell culture experiments are the 2in1 inserts suitable?
A: They are used in the production of skin models, 3D epithelial cultures, chemotaxis studies, transmigration assays, and transport and polarity studies.
Sterility and Quality
Q: What is the sterility level of the 2in1 inserts?
A: They have a Sterility Assurance Level (SAL) of 10-6 and are BIO-CERT® CELL CULTURE QUALITY certified.
Packaging and Handling
Q: How are the inserts packaged?
A: They are available either individually packaged or in convenient multi-packs, with the latter being able to be opened row by row to maintain the sterility of the remaining inserts.
Q: Are there any special considerations for handling PC membranes?
A: PC membranes may experience pore enlargement and fiber stretching when exposed to 100% DMSO. Prolonged contact may cause the membranes to wrinkle.
